Protein Purification Reagents and Kits
STEMCELL Technologies RoboSep™ Human Biotin Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553). For highly purified particle-free cells, we recommend the EasySep™ Release Human Biotin Positive Selection Kit (Catalog #17653). (https://www.stemcell.com/products/easysep-release-human-biotin-positive-selection-kit.html)
STEMCELL Technologies RoboSepTM Human Progenitor C
EasySep(TM) kit with RoboSep(TM) Buffer and RoboSep(TM) filter tips - 1 x 10.9 cells
Worthington Biochemical Corporation CELL ISOLATION OPTIMIZING SYS
A complete method development kit containing an assortment of enzymes most frequently used in tissue dissociation and cell isolation procedures. Includes instructions, references and strategies for the handling, use and optimization of enzymatic cell isolation methods to achieve maximum yield of viable cells. Kit includes 500 mg of each of four types of collagenase, 500 mg trypsin, 50 ku hyaluronidase, 100 mg elastase, 100 mg papain, 25 mg DNase I, 10 mg neutral protease (Dispase) and 100 mg trypsin inhibitor.
STEMCELL Technologies RoboSep™ Mouse Streptavidin RapidSpheres™ Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit is designed for the depletion of single or multiple unwanted cell types labeled with biotinylated antibodies. Labeled cells from mouse splenocytes or other tissues are targeted for removal by Streptavidin RapidSpheres™ and separated without the use of columns using an EasySep™ magnet. Desired cells are untouched and poured off into a new tube.
STEMCELL Technologies RoboSep™ Mouse B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-B cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using and EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For isolation of B cells expressing CD11b or CD43, we recommend using the EasySep™ Mouse Pan-B Cell Isolation kit (Catalog #19844 (https://www.stemcell.com/products/easysep-mouse-pan-b-cell-isolation-kit.html)).This product replaces the EasySep™ Mouse B Cell Enrichment Kit (Catalog #19754) for even faster cell isolations.
American Research Products Inc Rat Epithelial neutrophil acti
Rat Epithelial neutrophil actiVating peptide 78 ENA-78 ELISA Kit from CUSABIO Detection Range 0 312 ng/mL-20 ng/mL Sensitivity 0 078 ng/mL Sample serum plasma tissue homogenates Method Sandwich
STEMCELL Technologies RoboSepTM Mouse Hematopoieti
EasySep(TM) kit with RoboSep(TM) buffer and RoboSep(TM) filter tips - 1 x 10.9 cells
STEMCELL Technologies RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail, For processing: 40 mL cord blood processed
The RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail is designed to isolate progenitor cells from cord blood and whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD61, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified progenitor cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium. If using large volumes of blood, we recommend the RosetteSep™ Cord Blood Progenitor Enrichment Kit with HetaSep™ (Catalog #15276) (/products/rosettesep-human-cord-blood-progenitor-enrichment-kit.html).
Miltenyi Biotec, Inc. Adult Brain Dissociation Kit
Dissociation of adult brain tissue and debris removal for the subsequent isolation of neurons, astrocytes and oligodendrocytes
STEMCELL Technologies RoboSep™ Human NK Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human NK Cell Enrichment Kit is designed to isolate NK cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-NK cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For even faster cell isolations, we recommend the new EasySep™ Human NK Cell Isolation Kit (17955) (/products/easysep-human-nk-cell-isolation-kit.html) which isolates cells in just 8 minutes.
STEMCELL Technologies EasySep™ Rat T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Rat T Cell Isolation Kit is designed to isolate T cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies RoboSep™ Human CD8+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This product can be used in place of the EasySep™ Human CD8+ T Cell Enrichment Kit (Catalog #19053) for even faster cell isolations.
Biotium CD45RA (Leukocyte Marker)(K4B5), CF740 conjugate, 0.1mg/mL
Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4 T-cells, 50% of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO ve) from B cell lymphomas (CD45RA ve). Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other l
STEMCELL Technologies EasySep™ PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ PE Positive Selection Kit (Catalog #18557) for even faster cell isolations.